Notice for the Seminar of the Dept. of BIological Sciences
The department of Biological Science will hold the following seminar.
1. Date: 2019. 12. 5. (Thu.) 17:00 2. Vanue: Room No. 31317 (Institute of Bsic Science Seminar room) 3. Theme: Cycling DOF transcription factor determines photoperiodicresponse on secondary growth via direct control of cambium activity 4. Speaker: Prof. Hyun-woo Jo (Dept. of Industrial Plant Science & Technology, College of Agriculture, Life & Environmental Sciences, Chungbuk National University) 5. Abstract: Day or night length sensing mechanism is a key invention for evolution of land plant to survive, reproduce and adapt environmental challenges that the sessile plant continuously faces. Consequent modification of physiological activities under photoperiodic input makes a direct contribution to their survival and reproduction through optimizing growth and development (active or dormant state) and timing of vegetative to reproductive phase transition (flowering time), respectively. In line with precise orchestration of photoperiodic response for plant fitness, stem cell ability continuously shaping the body in a plastic manner is a key strategy to cope with challenging environment. However, how plant integrates photoperiodic input into regulation of stem cell activity is largely unanswered. Herein, we show a regulatory unit for balancing cambium maintenance and differentiation under the control of photoperiod involving the CYCLING DOF FACTORs (CDFs) and PHLOEM INTERCALATED WITH XYLEM/TDIF RECEPTOR (PXY/TDR). The genome wide comparative analysis by combination of fluorescence-activated nuclei sorting (FANS)-assisted assay for transposase-accessible chromatin (ATAC) and FANS-assisted RNA-seq, we reveal cambium-specific open chromatin profile and potential role of CDF on these regions. Interestingly, large proportion of CDFs diurnally expressed in stem, which is controlled by photoperiodic pathway, negatively affect cambium activity by inhibiting PXY expression. In turn, decoupling of CDF expression from photoperiodic input and blocking of CDF binding to PXY promoter show severe defect in secondary growth. In summary, our study delineates CDF-PXY signaling module that regulates photoperiodic control of secondary growth.